Gram 100 tests
    Cod. 04-100802

    Gram 100 tests

    Product for the preparation of cyto-histological samples for optical microscopy. To differentiate between Gram-positive and Gram-negative bacteria in tissue sections.

    €124.00
    Tax excluded
    Packaging
    • 1 kit
    Quantity
      Available

    A maximum of 5 pieces can be ordered

    DESCRIPTION

    Gram staining is the most important method to differentiate bacteria species. Two dyes are used one after the other: crystal violet and fuchsine. Crystal violet solution precipitates through oxidation with a iodine solution. The deriving complex attaches to bacteria cell walls with bonds of varying nature and intensity. The differentiating solution removes the crystal violet-iodine complex from the walls of some bacteria, but it does not act on others. These retain the primary dye and are called Grampositive. Decolorized bacteria are then counterstained with a red dye; they are called Gram-negative. Gram-positive bacteria’s capacity to retain the dye-iodine complex is usually ascribed to the bond which develops between the complex and a molecule only Gram-positive possess, namely magnesium ribonucleate.

    Gram-positive bacteria: blue.
    Gram-negative bacteria: red.
    Nuclei: red

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    INSTRUCTIONS FOR USE

    Instruction for use Alcian blue pH 2,5 100 tests
    • Bring section to distilled water.
    • Pour the content of bottle A in a Coplin Jar, introduce the slide and incubate at 56-58°C for 15 minutes; pour back the solution in bottle A filtering through filter paper.
    • Wash in distilled water.
    • Put on the section 10 drops of reagent B: leave to act 3 minutes.
    • Drain the slide without washing and put on the section 10 drops of reagent C: leave to act 3 minutes.
    • Wash in distilled water and dry the slide in filter paper then in the air for 10 minutes.
    • Pour the content of bottle D in a Coplin Jar: shake the slide for 1 minute; pour back the solution in bottle D filtering through filter paper.
    • Repeat step 7 with reagent E.
    • Clear in xylene and mount.

    DESCRIPTION

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    INSTRUCTIONS FOR USE

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    INSTRUCTIONS FOR USE

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    OTHER PRODUCTS

    We are an Italian company with the goal of ensuring everyone's most valuable asset: the health.

    Made in Italy High
    Quality Products.

    45 years of experience
    in anatomic pathology.

    ISO 13485 Certification
    & IVDR Compliant.

    Constant research
    for innovation.